Nickel has been extensively used as a high work function metal because of its abundance, low cost, relatively non-toxic nature, and environmentally benign characteristics. However, it has rarely been extended in a form of immobilized composite, which is a practical strategy applicable for photocatalytic antimicrobial activities. In this study, a composite of nickel and TiO2 (Ni/TiO2) was prepared using a photodeposition method, and its antibacterial properties were investigated using Escherichia coli (E. coli). To optimize Ni/TiO2 synthesis, the effect of various photodeposition conditions on antibacterial performance were investigated, such as the light irradiation time, metal content, TiO2 crystalline structure, and presence or absence of electron donors (i.e., methanol). The optimized 2 wt% Ni/TiO2 exhibited an antibacterial efficiency of 3.74 log within 7 min, which is more than 10-fold higher than that of pristine TiO2 (2.54 log). Based on this optimized weight ratio, Ni/TiO2 was immobilized on a steel mesh using an electrospray/thermal compression method, and its antibacterial performance was further assessed against E. coli, MS2 bacteriophage virus (MS2 phage), and a common pulmonary pathogen (Legionella thermalis, L. thermalis). Within 70 min, all target microorganisms achieved an inactivation that exceeded 4 log. Furthermore, the long-term stability and sustainable usability of the Ni/TiO2 mesh were confirmed by performing more than 50 antibacterial evaluation cycles using E. coli. The results of this study facilitate the successful utilization of immobilized Ni/TiO2 mesh in water disinfection applications.